![]() ![]() Urdangarin quickly learned that in this new stage of his life he was not going to have trouble finding partners or business opportunities. It was particularly curious given that he was still playing handball during that first year of study. According to an investigation by the daily ARA, it was thanks to unusual validations of previous studies that Urdangarin received a bachelor’s and master’s degree in Business Administration. He finished the course in two years, between 19, even though it normally takes five. But there were indications something was amiss. He began his reinvention by studying at the prestigious ESADE business school. This is the role I want to take on.” He wanted to be a champion in real life as well, which proved to be no small feat. I want to think about my professional future and I will fight so that this new stage will be as successful as the one I am leaving behind. When he left his club, Barcelona, he said: “You have to know how to say goodbye. Although he was used to the routine of an elite athlete, Urdangarin was optimistic about his future. ![]() He was no longer a champion, but a duke – at that time, he still didn’t know what that meant. It was a world he enjoyed, but one that also poisoned him. Urdangarin abandoned the sporting world he knew so well to become part of one that was much more complex, and involved power, aristocracy and privilege. I’ve learned about them as I went along,” he told Judge José Castro years later, while being questioned in court over his business dealings. “They haven’t explained a lot of things to me. All rights reserved.Urdangarin quickly learned that in this new stage of his life he was not going to have trouble finding partners or business opportunities. A RSD value of 6% was obtained for measurements carried out with 3 different genosensors prepared in the same manner.Īsymmetric polymerase chain reaction Electrochemical detection Genosensor Nanopatterning Rough gold electrode.Ĭopyright © 2013 Elsevier B.V. After optimization of the different variables involved in the hybridization and detection reactions, detection limits of 5.30 pg μL(-1) and 10 pg μL(-1) were obtained for the 25-mer synthetic target DNA and the aPCR amplicon, respectively. The hybridization event was electrochemically monitored by using two different indicators, hexaammineruthenium (III) chloride showing an electrostatic DNA binding mode, and pentaamineruthenium- (in brief RuL) which binds to double stranded DNA (dsDNA) following an intercalative mechanism. A 25 bases synthetic sequence fully complementary to the thiolated DNA capture probe and a 326 bases fragment of lacZ containing a fully matched sequence with the capture probe, which was amplified by a specific asymmetric polymerase chain reaction (aPCR), were employed as target sequences. A synthetic 25-mer DNA capture probe, modified at the 5' end with a hexaalkylthiol, able to hybridize with a specific sequence of lacZ gene from the Enterobacteriaceae bacterial family was assembled to the rough gold surface. The electrode surface nanopatterning was accomplished by repetitive square-wave perturbing potential (RSWPP). An electrochemical DNA genosensor constructed by using rough gold as electrode support is reported in this work. ![]()
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